Journal: bioRxiv
Article Title: HIWI2 Influences Endosomal Trafficking and Eph Receptor Signaling in Photoreceptor Cells
doi: 10.64898/2026.03.09.710476
Figure Lengend Snippet: Knockdown of HIWI2 in 661W cells was checked by Western blot and quantified (A,F). Western blot analysis and quantification of early endosomal protein Rab5 (B,G), early endosomal antigen 1 (EEA1) (C, H), recycling endosomal protein Rab11 (D,I), late endosomal protein Rab7 (E,J). Graph pad prism software was used for quantification. The students’ t-test was used for statistical analysis. Values are means ± SEM, n = 3. *p < 0.05 and **p < 0.01, ***p < 0.001 were considered statistically significant. Original blots for and are given in supplementary (Fig.S1A, B, C and D).
Article Snippet: The blots were incubated in blocking buffer (5% BSA) for 1 h and were probed against HIWI2 (Abcam), EphrinA2-total (CST), EphrinA2-phospho (CST), EphrinB2-total (CST), EphrinB2-phospho (Thermo Scientific), Rab5 (CST), Rab11 (CST), Early Endosome antigen-1 (CST), Rab 7 (CST), Actin (Sigma), GAPDH (CST), primary antibodies in a 1:1000 dilution.
Techniques: Knockdown, Western Blot, Software